Jobbeschreibung
The University Hospital Bonn (UKB) is a maximum care hospital with more than 1,300 beds. Our more than 9,000 employees take on tasks in research, teaching and patient care as well as in public health at the highest level. In the science ranking (LOMV) and in the economic result, the UKB is the number one of the university hospitals in NRW, and in 2021 it had the third-highest case mix index of the university hospitals in Germany.
At the Institute of Biochemistry and Molecular Biology, University of Bonn Medical School, Germany is available a part-time position (25.025 hrs/week):
PhD student (m/f/d)
Impaired protein homeostasis (proteostasis) frequently results in the accumulation of protein aggregates, a hallmark of neurodegenerative diseases. Aggregation is often a consequence of impaired folding, defects in complex assembly or failed sorting of proteins. After synthesis, about two thirds of the proteins have to be transported to different cellular compartments during or after synthesis in the cytosol. Impaired sorting results in cytosolic accumulation of such proteins, causing proteotoxic stress and an induction of the cellular stress response. We study the targeting of proteins to mitochondria and their cytosolic quality control when import fails (Paasch et al., 2018, Journal of Biological Chemistry; Schulte et al., 2023 Nature). Using genetic, cell biological, biochemical and proteomic analysis we observed targeting of mitochondrial proteins into the nucleus, where they are subjected to quality control (den Brave et al., Cell reports 2020). In our group, we monitor the fate of non-imported mitochondrial proteins by analyzing their stability, sorting, aggregation and post-translational modification by ubiquitin and ubiquitin-like proteins. In the frame of the DFG-funded priority program SPP2453, we will investigate the role of the nucleus as a central hub in the quality control of mitochondrial proteins.
We use highly innovative techniques in biochemistry, molecular and cell biology. This includes robot-based genetic screens combined with fluorescent imaging and protein-protein-interaction studies coupled with proteomics activity assays. We study the functionality and fate of proteins using protein stability, aggregation, disaggregation assays and analyse the state of post-translational modifications with ubiquitin-family proteins. We offer a well-equipped workspace in an active international team. The University of Bonn provides an excellent scientific research environment.
The position starts as soon as possible and is for a period of three years until 31.08.2027 due the project, with the possibility of an extension.
The ideal candidate will be a highly motivated, team-oriented graduate with a strong interest in protein biochemistry, molecular and cell biology. Experience with biochemical, cell and molecular biology techniques, microscopy or proteomics is advantageous. Candidates should hold a diploma or a master's degree in Biology, Biochemistry, Molecular Medicine, Chemistry or a related subject.
- The salary will be according to the German salary scale TV-L E13 65%
- Supportive mentoring and a well-equipped laboratory
- Responsible and versatile: A job with a lot of creative freedom in a collegial team
- Secure in the future: Fee according to TV-L
- Flexible for families: Flexible working time models, the option of a place in the company day-care center and offers for those returning from parental leave
- Provision for later: Company pension scheme
- Clever to work: Possibility of an interest-free loan to buy an e-bike
- Tailor-made education: Subsidized further education and training
- Systematic start: Structured induction
- Healthy at work: Numerous health promotion offers, free membership in UKBfit
- Employer benefits: Preferential offers for employees (corporate benefits)
The University of Bonn is committed to diversity and equal opportunity. It is certified as a family-friendly university. It aims to increase the proportion of women in areas where women are under-represented and to promote their careers in particular. It therefore urges women with relevant qualifications to apply. Applications will be handled in accordance with the Landesgleichstellungsgesetz (State Equality Act). Applications from suitable individuals with a certified serious disability and those of equal status are particularly welcome.
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